bromophenol blue
Definition
Noun: A synthetic dye belonging to the triphenylmethane family, used primarily as an acid-base indicator in analytical chemistry. It changes color depending on the pH of the solution.
Usage
Bromophenol blue is used to visually determine the acidity or alkalinity (pH) of a solution. It is commonly employed in titrations and in gel electrophoresis to track the migration of molecules.
Examples
- In a laboratory procedure:
- In a research context:
Advanced Usage
- As a tracking dye: In molecular biology, bromophenol blue is often mixed with dense substances like glycerol or sucrose and added to DNA samples. This allows researchers to see the sample during gel electrophoresis and estimate how far the DNA has migrated.
- pH Indicator range: Bromophenol blue exhibits a color change from yellow (in acidic conditions, below pH 3.0) to blue (in basic conditions, above pH 4.6).
Variants and Related Words
- Bromophenol (noun): A class of chemical compounds derived from phenol containing bromine atoms. Bromophenol blue is a specific member of this class.
- pH indicator (noun): A general term for any substance, like bromophenol blue, that changes color in response to pH.
- Tracking dye (noun): A dye, such as bromophenol blue or xylene cyanol, used to monitor progress in electrophoresis.
Synonyms
- Acid-base indicator (general term)
- pH dye (general term)
- BPB (common abbreviation in laboratory protocols)
Related Phrases/Idioms
(This term is technical and does not have idiomatic uses in everyday language. Its usage is confined to scientific contexts.)
Noun
-
a dye used as an acid-base indicator
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